Screening of extraction methods for glycoproteins from jellyfish (Rhopilema esculentum) oral-arms by high performance liquid chromatography

In order to select an optimum extraction method for the target glycoprotein (TGP) from jellyfish (Rhopilema esculentum) oral-arms, a high performance liquid chromatography (HPLC)-assay for the determination of the TGP was developed. Purified target glycoprotein was taken as a standard glycoprotein. The results showed that the calibration curves for peak area plotted against concentration for TGP were linear (r = 0.9984, y = 4.5895x+47.601) over concentrations ranging from 50 to 400 mgL-1. The mean extraction recovery was 97.84% (CV2.60%). The fractions containing TGP were isolated from jellyfish (R. esculentum) oral-arms by four extraction methods: 1) water extraction (WE), 2) phosphate buffer solution (PBS) extraction (PE), 3) ultrasound-assisted water extraction (UA-WE), 4) ultrasound-assisted PBS extraction (UA-PE). The lyophilized extract was dissolved in Milli-Q water and analyzed directly on a short TSK-GEL G4000PWXL (7.8 mm×300 mm) column. Our results indicated that the UA-PE method was the optimum extraction method selected by HPLC.     

The scavenging of free radical and oxygen species activities and hydration capacity of collagen hydrolysates from walleye pollock (Theragra chalcogramma) skin

Fish skin collagen hydrolysates (FSCH) were prepared from walleye pollock (Theragra chalcogramma) using a mixture of enzymes, namely trypsin and flavourzyme. The degree of hydrolysis of the skin collagen was 27.3%. FSCH was mainly composed of low-molecular-weight peptides and the relative proportion of <1000Da fraction was 70.6%. Free radical and oxygen species scavenging activities of FSCH were investigated in four model systems, including diphenylpicrylhy-drazyl radical (DPPH), superoxide anion radical, hydroxyl radical and hydrogen peroxide model, and compared with that of a native antioxidant, reduced glutathione (GSH). FSCH was also evaluated by water-absorbing and water-holding capacity. The results showed that FSCH was able to scavenge free radical and oxygen species significantly and to enhance water-absorbing and water-holding capacity remarkably. Therefore, FSCH may have potential applications in the medicine and food industries.     

Soil evolution features of debris flow waste-shoal land

The reclamation and utilization of debris flow waste-shoal land plays an important role in the mitigation and control of debris flow hazards, which thus contributes a lot to the exploitation of insufficient land resources in mountainous areas and the reduction of losses caused by debris flow. The aim of this paper is to discuss the features and mechanism of soil evolution of debris flow waste-shoal land so as to search for the available modes of its reclamation and utilization. The Jiangjiagou Ravine, a typical debris flow ravine, was selected to study soil evolution features of debris flow waste-shoal land based on the analysis of soil physieochemical properties and soil microstructure. It was found that the soil evolution rates of debris flow waste-shoal land varied with different modes of reclamation. For the land which had been reclaimed for less than lO years, soil evolved most rapidly in paddy fields, and more rapidly in dry farmland than in naturally restored waste-shoal land. For the land which had been used for more than lo years, the soil evolution rates of dry farmland, naturally restored waste-shoal land and paddy farmland decreased in the file. For the same utilization period of time, significant differences were recognized in soil evolution features under different modes of reclamation. Analysis data showed that soil clay content, soil thickness, the psephicity of skeleton particles and contents of microaggregates （〈0.02 mm） in paddy farmland were all highest. Soil nutrients and porosity of dry farmland were better than those of paddy farmland and naturally restored waste-shoal land, and those of paddy farmland were superior to those of naturally restored waste-shoal land. Paddy farmland characterized by rapid pedogenesis, stable evolution and high utilizability was the priority candidate for the reclamation and utilization of debris flow waste -shoal land.     

加拿大海盆北部营养盐限制作用研究

利用2008年夏季中国第三次北极科学考察获得的营养盐、叶绿素a、温度和盐度等数据资料,结合现场营养盐添加实验的结果讨论西北冰洋加拿大海盆北部营养盐对浮游植物生长的限制作用。结果表明:由于融冰水稀释作用,加拿大海盆B80站约20m深度存在较强的盐跃层,阻碍了水体上下混合。较低浓度的溶解无机氮(DIN)和硅酸盐(分别为0.31μmol/L和0.94μmol/L)以及严重偏离Redfield比值的N/P、N/Si比值(分别为0.42和0.32)表明加拿大海盆表层水体存在N和Si限制。根据现场营养盐加富实验各培养组叶绿素a浓度变化、营养盐吸收总量差异和浮游植物种群结构,进一步表明氮是北冰洋海盆首要限制营养盐,而Si则抑制了硅质生物的生长。同时,较小的硝酸盐半饱和常数(Ks)证明即使在营养盐充足的情况下北冰洋海盆浮游植物生长速率也处于较低水平。计算得到各培养组营养盐吸收比例(N/P比值)均大于Redfield比值,可能是培养实验过程中以微型、微微型浮游植物为主,硅藻等小型浮游植物为辅造成的。     

多时相Sentinel-1A InSAR的连盐高铁沉降监测分析

多时相InSAR技术具有探测大范围毫米级地表形变的能力,已被广泛应用于地面沉降监测.近几年,应用多时相InSAR技术监测以高铁为代表的大尺度人造线状地物形变备受关注.本文将C波段SAR数据用于高铁沿线路基形变监测,应用相位稳定性分析和改进的StaMPS技术来增加相干性点的密度和形变参数解算的稳定性.采用研究区时间跨度为21个月的47景Sentinel-1A数据,对连(云港)盐(城)高铁及其沿线区域进行多时相InSAR分析,并利用同时期连续的北斗导航卫星系统(BeiDou navigation satellite system,BDS)观测数据对线性沉降速率和时序位移分别进行监测分析,进而对比验证二者的沉降监测结果.研究结果表明:利用C波段的Sentinel-1A数据能够获取毫米级的线性形变速率和时序位移序列,InSAR与BDS二者平均时序位移均方根误差为3.8 mm,与BDS监测结果相比取得很好的一致性,且连盐高铁线路整体表现稳定.     

基于简译的高分辨率卫星影像信息提取研究

利用简译遥感信息提取软件,以资源三号高分辨率卫星影像为数据源,选取老挝首都万象市为研究区,采用分层信息提取思想,在道路、建筑等地表覆盖要素人工提取结果的基础上,对未分类地物(水田、旱地、林地、工矿用地),利用最小距离算法和深度学习算法进行信息提取研究.研究结果表明,基于最小距离算法的总体分类精度为85.7％,Kappa系数为0.8165;基于深度学习算法的总体分类精度为89.29％,Kappa系数为0.8634,都可以达到很好的智能化提取效果.     

BJ-RUC产品在小区供暖供回水温度预报的应用研究

利用气象部门快速更新的BJ-RUC(Beijing-Rapid UpdateCycle)数值预报系统获得的24 h逐时的室外温度预报资料,应用能量平衡原理,建立建筑物热损失方程和供热量方程,从而推导出室外温度和室内温度、供水、回水温度的预报模型。用此模型对2010年3月1—15日期间的供、回水温度进行预报模拟,与北京市海园物业供热单位实况值进行对比分析。检验结果表明,该模型模拟的室内温度与设计要求的室内温度(20℃)之间的均方根误差为0.25℃,海园物业室内温度监测点数值与20℃之间的均方根误差为1.53℃。模拟的供、回水温度与海园物业监测点供水温度的均方根误差分别为3.69℃和3.80℃。     

基于模糊评判的数控机床零部件制造工艺FMECA研究

以数控机床零部件的制造工艺为研究对象,对其实施改进的制造工艺FMECA,通过梯形模糊数评判的方法确定出工艺FMECA中风险优先数的排序,利用模糊综合评判方法确定出工艺系统的风险等级,有效地找出薄弱工艺环节并采取改进措施,从而提高数控机床零部件制造工艺可靠性.     

GEO空间碎片光度测量标定方法的研究

基于GEO卫星、太阳和观测站的空间几何关系,建立了消除太阳赤纬角对地球同步轨道( Geostationary Orbit,GEO)卫星光学特性的影响的方法.以三轴稳定GEO盒状卫星缩比模型为研究样本,在不同太阳赤纬角下进行仿真实验光度测量,研究太阳赤纬角和相位角的空间几何关系,得出消除太阳赤纬角对其辐亮度值以及以相位角...     

鲁西新泰孟家屯2.7Ga变质沉积岩与黑云斜长片麻岩锆石Hf同位素特征

对出露于山东新泰孟家屯2.7Ga的孟家屯岩组中石榴石石英岩(M08)、含十字石石榴石黑云母石英片岩(D242-Y2)和黑云斜长片麻岩(M03)(TTG质片麻岩)进行锆石Lu-Hf同位素分析。石榴石石英岩锆石核部176Lu/177Hf、176Hf/177Hf变化范围为0.001730～0.002546、0.281249～0.281360,锆石变质边部176Lu/177Hf、176Hf/177Hf变化范围为0.000123～0.002070、0.281241～0.281318;含十字石石榴石黑云母石英片岩锆石核部176Lu/177Hf、176Hf/177Hf变化范围为0.001334～0.002169、0.281226～0.281324,锆石变质边部176Lu/177Hf、176Hf/177Hf变化范围为0.000445～0.001375、0.281227～0.281309;黑云斜长片麻岩锆石176Lu/177Hf、176Hf/177Hf变化范围为0.000315～0.000847、0.281186～0.281265。孟家屯岩组石榴石英岩、含十字石石榴石黑去母石英片岩中碎屑(岩浆)锆石和黑云斜长片麻岩中岩浆锆石的εHf(t)分别为3.75～7.26、2.31～7.26和3.21～6.27,同时TDM1与其U-Pb年龄非常接近,表明它们起源于新生地壳的部分熔融。结合区域研究资料认为,2.7Ga是鲁西重要的一期构造岩浆热事件,也可能是华北克拉通重要的构造岩浆活动时期。变质沉积岩中许多变质增生锆石相对于核部岩浆锆石低176Lu/177Hf、高176Hf/177Hf,显示变质作用过程中石榴石的存在对锆石的Lu-Hf同位素体系有很大影响。